Aflatoxin Determination in Forages and Feeds

Quantitative determination of Aflatoxins (B1, B2, G1, G2) by LC-MS/MS. Aflatoxins are carcinogenic and potent liver toxins. Aflatoxin exposure causes rough hair, lethargy and immune suppression.
Taking a representative sample:
The analytical result is only as good as the sample submitted for analysis. Collecting a representative sample is the first step of the analytical procedure.
  • Hay & haylage: Different types, cuttings or lots should be analysed separately. Take at least a handful from at least 5 bales selected at random. Combine all the samples and submit as one sample for analysis. The sample should weigh at least 100g.
  • Grass: Randomly select between 15–20 sites on a ‘W’ formation across the field where the animals have been grazing and clip at grazing height. Combine in a clean plastic bucket and mix. Take at least 500g and pack tightly in a plastic bag and submit sample as fresh as possible.
  • Grains & mixed feeds: Bulk loads: Randomly collect around 10 samples as the grain is discharged and combine in a clean plastic bucket. Submit at least 500g for analysis.
  • Bags: Collect samples from as many different areas of the bag(s) as possible, mixing if possible to ensure a good representative sample is taken. Submit at least 100g for analysis.
Sample Requirement Turnaround Time Price Laboratory Species
Representative sample in a large ziploc bag 10 days €50.00 Environment & Nutrition All

Reference Ranges

Recommended upper limit: 5 µg/kg
*as per EU Directive

Related test(s)

Fungal count and enumeration, Mycotoxin analysis

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