Quantitative determination of Aflatoxins (B1, B2, G1, G2) by LC-MS/MS. Aflatoxins are carcinogenic and potent liver toxins. Aflatoxin exposure causes rough hair, lethargy and immune suppression.
Taking a representative sample:
The analytical result is only as good as the sample submitted for analysis. Collecting a representative sample is the first step of the analytical procedure.
Hay & haylage: Different types, cuttings or lots should be analysed separately. Take at least a handful from at least 5 bales selected at random. Combine all the samples and submit as one sample for analysis. The sample should weigh at least 100g.
Randomly select between 15–20 sites on a ‘W’ formation across the field where the animals have been grazing and clip at grazing height. Combine in a clean plastic bucket and mix. Take at least 500g and pack tightly in a plastic bag and submit sample as fresh as possible.
Grains & mixed feeds: Bulk loads: Randomly collect around 10 samples as the grain is discharged and combine in a clean plastic bucket. Submit at least 500g for analysis.
Bags: Collect samples from as many different areas of the bag(s) as possible, mixing if possible to ensure a good representative sample is taken. Submit at least 100g for analysis.
Representative sample in a large ziploc bag
Environment & Nutrition
Recommended upper limit: 5 µg/kg
*as per EU Directive
Fungal count and enumeration, Mycotoxin analysis
Irish Equine Centre, Johnstown, Naas, County Kildare, Ireland. Eircode: W91 RH93