Quantitative determination of T-2 toxin & HT-2 by LC-MS/MS
Exposure to T-2 causes gut upset, vomiting, diarrhoea, bowel inflammation, immune suppression and weight loss. These toxins are heat resistant and therefore resistant to cooking processes.

Taking a representative sample:
The analytical result is only as good as the sample submitted for analysis. Collecting a representative sample is the first step of the analytical procedure.
Hay & Haylage: Different types, cuttings or lots should be analysed separately. Take at least a handful from at least 5 bales selected at random. Combine all the samples and submit as one sample for analysis. The sample should weigh at least 100g.
Grass: Randomly select between 15 –20 sites on a ‘W’ formation across the field where the animals have been grazing and clip at grazing height. Combine in a clean plastic bucket and mix. Take at least 500g and pack tightly in a plastic bag and submit sample as fresh as possible.
Grains & Mixed Feeds: Bulk loads: Randomly collect around 10 samples as the grain is discharged and combine in a clean plastic bucket. Submit at least 500 g for analysis. Bags: collect grab samples from as many different areas of the bag(s) as possible, mixing if possible to ensure a good representative sample is taken. Submit at least 100g for analysis